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Antifungal Assay Agar
山东拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
Antifungal Assay Agar
Composition per liter:
Glucose.......................................................................................50.0g
Agar............................................................................................15.0g
Sodium citrate...............................................................................4.5g
Pancreatic digest of casein............................................................4.0g
Citric acid......................................................................................1.0g
K2HPO4 .......................................................................................0.55g
KCl............................................................................................0.425g
CaCl2 ·2H2O ..............................................................................0.125g
MgSO4·7H2O............................................................................0.125g
Inositol......................................................................................0.025g
MnSO4·4H2O.............................................................................2.5mg
Niacin.........................................................................................2.5mg
Caclium pantothenate ................................................................2.5mg
FeCl3 ..........................................................................................2.5mg
Pyridoxine hydrochloride........................................................0.25mg
Thiamine..................................................................................0.25mg
Biotin.....................................................................................0.008mg
pH 5.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Sigma
Aldrich.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For assaying antifungal activity of pharmaceutical products and
other materials by cylinder plate or disc method.