Antifungal Assay HiVeg Agar

[所属分类:培养基配方] [发布时间:2019-11-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antifungal Assay HiVeg Agar
Composition per liter:
Glucose.......................................................................................50.0g
Agar............................................................................................15.0g
Sodium citrate...............................................................................4.5g
Plant hydrolysate...........................................................................4.0g
Citric acid......................................................................................1.0g
K2HPO4 .......................................................................................0.55g
KCl............................................................................................0.425g
CaCl2·2H2O...............................................................................0.125g
MgSO4·7H2O............................................................................0.125g
Inositol......................................................................................0.025g
MnSO4·4H2O.............................................................................2.5mg
Niacin.........................................................................................2.5mg
Calcium pantothenate ................................................................2.5mg
FeCl3 ..........................................................................................2.5mg
Pyridoxine hydrochloride ........................................................0.25mg
Thiamine..................................................................................0.25mg
Biotin .....................................................................................0.008mg
pH 5.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For assaying antifungal activity of pharmaceutical products and
other materials by cylinder plate or disc method.