Antimicrobial Inhibitor Test Agar pH 7.2

[所属分类:培养基配方] [发布时间:2019-11-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antimicrobial Inhibitor Test Agar pH 7.2
Composition per liter:
Agar ............................................................................................13.0g
Peptone..........................................................................................7.0g
NaCl..............................................................................................5.0g
Na3PO4·12H2O..............................................................................0.8g
Selective supplement solution .................................................10.0mL
Bacillus subtilis spore suspension..............................................1.0mL
pH 7.2 ± 0.2 at 25°C
Selective Supplement Solution:
Composition per 10.0mL:
Trimethoprim.............................................................................5.0mg
Preparation of Selective Supplement Solution: Add trimethop-
rim to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium:  Add components, except selective sup-
plement solution and Bacillus subtilis spore suspension, to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to
7.2. Gently heat and bring to boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Add se-
lective supplement solution and Bacillus subtilis spore suspension.
Mix thoroughly. Pour into sterile Petri dishes.
Use:  For residual analysis of antimicrobial components in meat and
organ samples, using Bacillus subtilis ATCC 6633 as test organism.