Antimicrobial Inhibitor Test Agar pH 8.0

[所属分类:培养基配方] [发布时间:2019-11-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antimicrobial Inhibitor Test Agar pH 8.0
Composition per liter:
Tryptone........................................................................................3.5g
Meat extract..................................................................................3.5g
NaCl..............................................................................................5.0g
Na3PO4·12H2O .............................................................................2.5g
Agar............................................................................................13.0g
Micrococcus luteus suspension................................................10.0mL
Bacillus subtilis spore suspension .............................................1.0mL
pH 8.0 ± 0.2 at 25°C
Preparation of Medium:  Add components, except Bacillus subtilis
spore suspension, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Adjust pH to 8.0. Gently heat and bring to
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Add Bacillus subtilis spore suspension
and Micrococcus luteus (10 4 CFU per mL) suspension. Mix thorough-
ly. Pour into sterile Petri dishes.
Use:  For residual analysis of antimicrobial components in meat and
organ samples, using Bacillus subtilis ATCC 6633 and Micrococcus
luteus ATCC 9341 as test organisms.