ASM Medium

[所属分类:培养基配方] [发布时间:2019-11-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ASM Medium
Composition per 1001.2mL:
Na2HPO4 ................................................................................866.0mg
NH4·Cl ...................................................................................535.0mg
KH2PO4 ..................................................................................531.0mg
K2SO4 ....................................................................................174.0mg
MgSO4 ·7H2O...........................................................................37.0mg
CaCl2 ·2H O..............................................................................7.35mg
Trace elements solution .............................................................1.0mL
FeSO 4  solution...........................................................................0.2mL
Trace Elements Solution:
Composition per liter:
ZnSO4 ·7H2O..........................................................................288.0mg
MnSO4 ·4H2O.........................................................................224.0mg
CuSO4 ·5H2O..........................................................................125.0mg
KI.............................................................................................83.0mg
H3BO3 ......................................................................................61.8mg
Na2MoO4 ·2H2O .......................................................................48.4mg
CoCl2 ·6H2O .............................................................................47.6mg
H2SO4 , 1M.................................................................................1.0mL
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
FeSO 4 Solution:
Composition per liter:
FeSO4 ·7H2O ..........................................................................278.0mg
Preparation of FeSO 4 Solution:  Add FeSO 4 ·7H 2 O to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except trace elements
solution and FeSO 4  solution, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 1.0mL of trace elements solution and 0.2mL of
sterile FeSO 4  solution. Mix thoroughly. Aseptically distribute into ster-
ile tubes or flasks.
Use: For the cultivation of Mycobacterium species.