ASN-III Agar

[所属分类:培养基配方] [发布时间:2019-11-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ASN-III Agar
Composition per liter:
NaCl............................................................................................25.0g
MgSO4 ·7H2O................................................................................3.5g
MgCl2 ·6H2O..................................................................................2.0g
NaNO3 .........................................................................................0.75g
K2HPO4 ·3H2O.............................................................................0.75g
CaCl2 ·2H2O...................................................................................0.5g
KCl................................................................................................0.5g
Na2CO3 .......................................................................................0.02g
Citric acid...................................................................................3.0mg
Ferric ammonium citrate............................................................3.0mg
Magnesium EDTA.....................................................................0.5mg
Vitamin B 12 ...............................................................................10.0μg
Agar solution..........................................................................100.0mL
A-5 trace metals.........................................................................1.0mL
pH 7.3 ± 0.2 at 25°C
Agar Solution:
Composition per 100.0mL:
Noble agar...................................................................................10.0g
Preparation of Agar Solution: Add agar to glass-distilled water and
bring volume to 100.0mL. Mix thoroughly. Gently heat and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
A-5 Trace Metals:
Composition per liter:
H3BO3 .........................................................................................2.86g
MnCl2 ·4H2O................................................................................1.81g
ZnSO4 ·7H2O.............................................................................0.222g
CuSO4 ·5H2O.............................................................................0.079g
Co(NO3)2·6H2O........................................................................0.049g
Na2MoO4 ·2H2O........................................................................0.039g
Preparation of A-5 Trace Metals: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except agar solution, to
glass-distilled water and bring volume to 900.0mL. Mix well and heat
gently until dissolved. Filter sterilize. Warm to 45°–50°C. Aseptically
add agar solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the cultivation of Xenococcus species. For the isolation of
cyanobacteria from marine habitats.