ASN-III Broth

[所属分类:培养基配方] [发布时间:2019-11-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ASN-III Broth
Composition per liter:
NaCl............................................................................................25.0g
MgSO4 ·7H2O................................................................................3.5g
MgCl2 ·6H2O .................................................................................2.0g
NaNO3 ........................................................................................0.75g
K2HPO4 ·3H2O ............................................................................0.75g
CaCl2 ·2H2 ..................................................................................0.5g
KCl................................................................................................0.5g
Na2CO3 .......................................................................................0.02g
Citric acid...................................................................................3.0mg
Ferric ammonium citrate............................................................3.0mg
Magnesium EDTA.....................................................................0.5mg
Vitamin B 12 ...............................................................................10.0μg
A-5 trace metals.........................................................................1.0mL
pH 7.3 ± 0.2 at 25°C
A-5 Trace Metals:
Composition per liter:
H3BO3 .........................................................................................2.86g
MnCl2 ·4H2O ...............................................................................1.81g
ZnSO4 ·7H2O.............................................................................0.222g
CuSO4 ·5H2O.............................................................................0.079g
Co(NO3) 2 ·6H2O........................................................................0.049g
Na2MoO4 ·2H2O........................................................................0.039g
Preparation of A-5 Trace Metals: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to glass-distilled water
and bring volume to 1.0L. Mix well and heat gently until dissolved. Fil-
ter sterilize.
Use: For the cultivation of Xenococcus species. For the isolation of
cyanobacteria from marine habitats.