ASP-2 Medium

[所属分类:培养基配方] [发布时间:2019-11-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ASP-2 Medium
Composition per liter:
NaCl............................................................................................18.0g
MgSO4 ·7H2O................................................................................5.0g
KCl................................................................................................0.6g
NaNO3 ........................................................................................0.05g
Trace elements solution...........................................................10.0mL
Tris buffer solution ....................................................................4.0mL
CaCl2 ·2H2O solution..................................................................2.8mL
Na2SiO3 ·9H2O solution .............................................................1.5mL
Vitamin solution.........................................................................1.0mL
K2HPO4 solution........................................................................0.5mL
Vitamin B 12 solution..................................................................0.1mL
pH 7.6 ± 0.2 at 25°C
CaCl2 ·2H2O Solution:
Composition per 100.0mL:
CaCl2 ·2H2O.................................................................................13.0g
Preparation of CaCl2 ·2H2O Solution: Add CaCl 2 ·2H2O to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
K 2 HPO 4 Solution:
Composition per 100.0mL:
K2HPO4 .........................................................................................1.0g
Preparation of K 2 HPO 4 Solution: Add K 2 HPO 4 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly.
Tris Buffer Solution:
Composition per 100.0mL:
Tris[hydroxymethyl]aminomethane buffer.................................25.0g
Preparation of Tris Buffer Solution: Add 25.0g of tris to 65.0mL of
distilled/deionized water. Titrate to pH 7.6–7.7 with concentrated HCl.
Bring to 100.0mL with distilled/deionized water. Recheck pH after 12 hr.
Vitamin B 12 Solution:
Composition per 100.0mL:
Vitamin B 12 ................................................................................2.0mg
Preparation of VitaminB 12 Solution: Add vitamin B 12  to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
EDTA............................................................................................3.0g
MnCl2 ·4H2O...........................................................................432.0mg
FeCl3 ·6H2O............................................................................384.0mg
H3BO3 ....................................................................................342.0mg
ZnCl2 ........................................................................................31.5mg
CoCl2 ·6H2O ...............................................................................2.0mg
CuClor CuCl2 ..........................................................................0.25mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per 100.0mL:
Inositol...................................................................................500.0mg
Thymine.................................................................................300.0mg
Thiamine·HCl ..........................................................................50.0mg
Calcium  D -(+)-pantothenate.....................................................10.0mg
Nicotinic acid...........................................................................10.0mg
PABA.........................................................................................1.0mg
Folic acid....................................................................................0.2mg
Biotin .........................................................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly.
Na2SiO3 ·9H2O Solution:
Composition per 100.0mL:
Na2SiO3 ·9H2O.............................................................................10.0g
Preparation of Na2SiO3 ·9H 2 O Solution: Add Na 2 SiO 3 ·9H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Asep-
tically distribute into sterile, screw-capped tubes or flasks.
Use: For the cultivation of Chlamydomonas species.