Asparagine Broth (Coccidioidin and Histoplasmin Broth)

[所属分类:培养基配方] [发布时间:2019-11-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Asparagine Broth
(Coccidioidin and Histoplasmin Broth)
Composition per liter:
Glucose.......................................................................................10.0g
L -Asparagine.................................................................................7.0g
NH4Cl ...........................................................................................7.0g
MgSO4 ·7H2O................................................................................1.5g
K2HPO4 .......................................................................................1.31g
Sodium citrate...............................................................................0.9g
Ferric citrate .................................................................................0.3g
Glycerol ...................................................................................25.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add glycerol to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Add remaining compo-
nents. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the preparation of the cultivation of Coccidioides and Histo-
plama for the production of coccidoidin and histoplasmin antigens for
immunologic work.