Asparagine Gelatin Lactate Medium Base with Lactate

[所属分类:培养基配方] [发布时间:2019-11-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Asparagine Gelatin Lactate Medium Base
with Lactate
Composition per liter:
Gelatin.......................................................................................150.0g
Lactate...........................................................................................5.0g
Asparagine....................................................................................1.0g
K2HPO4 .........................................................................................0.5g
MgSO4·7H2O................................................................................1.0g
FeNH4(SO4)2 ·12H2O .................................................................1.0mg
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix well until dissolved. Adjust pH
to between 6.9 and 7.2. Distribute into tubes or flasks. Autoclave for 15
min at 10 psi pressure–115°C. Pour into Petri dishes or leave in tubes
Use: For the isolutation of sulfur bacteria such as Desulfovibrio spp.