Aspergillus Medium

[所属分类:培养基配方] [发布时间:2019-11-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aspergillus Medium
Composition per liter:
Agar............................................................................................15.0g
NaNO3 ..........................................................................................6.0g
Casamino acids.............................................................................1.0g
Peptone .........................................................................................1.0g
Yeast extract..................................................................................1.0g
Adenine.......................................................................................0.15g
Vitamin solution.......................................................................10.0mL
pH 6.0 ± 0.2 at 25°C
Vitamin Solution:
Composition per 100.0mL:
Biotin..........................................................................................0.01g
Nicotinic acid..............................................................................0.01g
p-Aminobenzoic acid..................................................................0.01g
Pyridoxine·HCl...........................................................................0.01g
Riboflavin...................................................................................0.01g
Thiamine·HCl .............................................................................0.01g
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 10 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 6.0. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 10.0mL of sterile vitamin solution. Mix thoroughly. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Aspergillus amstelodami,
Aspergillus awamori, Aspergillus flavus, and Aspergillus nidulans.