Aspergillus nidulans Minimal Medium

[所属分类:培养基配方] [发布时间:2019-11-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aspergillus nidulans Minimal Medium
Composition per 950.0mL:
Solution A..............................................................................500.0mL
Solution B..............................................................................250.0mL
Solution C..............................................................................200.0mL
pH 6.5 ± 0.2 at 25°C
Solution A:
Composition per 500.0mL:
NaNO3 ...........................................................................................6.0g
KCl..............................................................................................1.52g
KH2PO4 .......................................................................................1.52g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 6.5
with 2N NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C.
Solution B:
Composition per 250.0mL:
Agar ............................................................................................15.0g
MgSO4 ·7H2O..............................................................................0.52g
FeSO4 ·7H2O................................................................................1.0μg
ZnSO4 ·7H2O...............................................................................1.0μg
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 250.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C.
Solution C:
Composition per 200.0mL:
Glucose .......................................................................................10.0g
Preparation of Solution C:  Add glucose to distilled/deionized wa-
ter and bring volume to 200.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°–55°C.
Preparation of Medium: Aseptically combine sterile solution A,
sterile solution B, and sterile solution C. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Aspergillus nidulans.