Aspergillus Test Medium

[所属分类:培养基配方] [发布时间:2019-11-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aspergillus Test Medium
Composition per liter:
Agar ............................................................................................20.0g
Malt extract.................................................................................20.0g
Peptone..........................................................................................1.0g
Glucose solution ....................................................................100.0mL
Supplement solution ..............................................................100.0mL
pH 6.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose .......................................................................................20.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize. Warm to 50°C.
Supplement Solution:
Composition per 100.0mL:
Uridine........................................................................................2.44g
Arginine .................................................................................200.0mg
Methionine...............................................................................50.0mg
Riboflavin ..................................................................................2.5mg
Nicotinic acid.............................................................................2.0mg
p-Aminobenzoic acid.................................................................1.0mg
Pyrdoxine·HCl .........................................................................0.05mg
Biotin .......................................................................................0.02mg
Preparation of Supplement Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize. Warm to 50°C.
Preparation of Medium: Add components, except glucose solution
and supplement solution, to distilled/deionized water and bring volume
to 800.0mL. Mix thoroughly. Adjust pH to 6.5. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C. Aseptically add 100.0mL of sterile glucose solution and
100.0mL of sterile supplement solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Emericella (Aspergillus)
nidulans.