Atlas Oil Agar

[所属分类:培养基配方] [发布时间:2019-11-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Atlas Oil Agar
Composition per liter:
Bushnell-Haas agar................................................................990.0mL
Oil ............................................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Bushnell-Haas Agar:
Composition per 990.0mL:
Agar ............................................................................................15.0g
KH2PO4 .........................................................................................1.0g
K2HPO4 .........................................................................................1.0g
NH4NO3 ........................................................................................1.0g
MgSO4 ·7H2O................................................................................0.2g
FeCl3 ...........................................................................................0.05g
CaCl2 ·2H2O.................................................................................0.02g
Preparation of Bushnell-Haas Agar: Add components to dis-
tilled/deionized water and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 60°C.
Preparation of Medium: Filter sterilize oil. Aseptically add
10.0mL of sterile oil to 990.0mL of cooled, sterile Bushnell-Haas agar.
Put mixture into a sterile blender container. Blend on low speed to min-
imize the incorporation of air into the medium. Pour into sterile Petri
dishes.
Use: For the cultivation and enumeration of hydrocarbon-utilizing
bacteria by direct plating of water and sediment samples.