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AT5N Medium
山东拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
AT5N Medium
Composition per liter:
CaCO 3 .........................................................................................10.0g
(NH4)2SO4 ....................................................................................1.5g
K2HPO4 .........................................................................................0.5g
MgSO4 .....................................................................................50.0mg
KHCO3 .....................................................................................30.0mg
CaCl2 ·2H2O .............................................................................20.0mg
Preparation of Medium: Add components to tap water and bring
volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of bacteria that oxidize ammonia, especially
those from wastewater.



