Aureobacterium terregens Medium

[所属分类:培养基配方] [发布时间:2019-11-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aureobacterium terregens Medium
Composition per liter:
Casamino acids.............................................................................2.0g
K2HPO4 .........................................................................................2.0g
Diammonium citrate.....................................................................1.0g
Glucose.........................................................................................1.0g
Yeast extract..................................................................................1.0g
MgSO4 ·7H2O................................................................................0.5g
FeCl3 ·6H2O..............................................................................10.0mg
Acetylacetone solution...............................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Acetylacetone Solution:
Composition per 100.0mL:
Acetylacetone .............................................................................10.0g
Ethanol (95% solution)..........................................................100.0mL
Preparation of Acetylacetone Solution: Add acetylacetone to
100.0mL of ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except acetylacetone
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature. Aseptically add 1.0mL of acetylacetone solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Aureobacterium terregens.