Aureomycin ® Rose Bengal Glucose Peptone Agar

[所属分类:培养基配方] [发布时间:2019-11-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aureomycin ® Rose Bengal
Glucose Peptone Agar
Composition per liter:
Agar............................................................................................20.0g
Glucose.......................................................................................10.0g
Peptone .........................................................................................5.0g
KH2PO4 .........................................................................................1.0g
MgSO4 ·7H2O................................................................................0.5g
Rose Bengal..............................................................................0.035g
Aureomycin solution .............................................................200.0mL
pH 5.4 ± 0.2 at 25°C
Aureomycin Solution:
Composition per 200.0mL:
Aureomycin·HCl.........................................................................0.07g
Preparation of Aureomycin Solution: Add aureomycin·HCl to
distilled/deionized water and bring volume to 200.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except aureomycin so-
lution, to distilled/deionized water and bring volume to 800.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 200.0mL of
sterile aureomycin solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and enumeration of fungi isolated from sew-
age and polluted waters.