Autotrophic Nitrobacter Medium (DSMZ Medium 756c)

[所属分类:培养基配方] [发布时间:2019-11-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Autotrophic Nitrobacter Medium
(DSMZ Medium 756c)
Composition per liter:
NaNO2 ...........................................................................................2.0g
Stock solution.........................................................................100.0mL
Trace elements solution .............................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Stock Solution:
Composition per liter:
NaCl..............................................................................................5.0g
KH2PO4 .........................................................................................1.5g
MgSO4 ·7H2O................................................................................0.5g
CaCO3 ........................................................................................0.07g
Preparation of Stock Solution: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
FeSO4 ·7H2O.............................................................................97.3mg
H3BO3 ......................................................................................49.4mg
ZnSO4 ·7H2O............................................................................43.1mg
(NH4)6Mo7O24 ·4H2O...............................................................37.1mg
MnSO4 ·2H2O...........................................................................33.8mg
CuSO4 ·5H2O............................................................................25.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.6. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Allow to stand for 2–3 days so that pH adjusts itself to 7.4–7.6.
Use:  For the cultivation of Nitrobacter winogradskyi.