Azide Blood Agar with Crystal Violet (Packer’s Agar)

[所属分类:培养基配方] [发布时间:2019-11-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azide Blood Agar with Crystal Violet
(Packer’s Agar)
Composition per liter:
Agar............................................................................................15.0g
Pancreatic digest of casein............................................................5.0g
Peptic digest of animal tissue .......................................................5.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
NaN 3 .............................................................................................0.9g
Crystal Violet.............................................................................2.0mg
Sheep blood, defibrinated........................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile
defibrinated sheep blood. Pour into sterile Petri dishes or distribute into
sterile tubes. Allow tubes to cool in a slanted position.
Use: For the isolation and enumeration of fecal streptococci from non-
clinical specimens such as water and food. Also used for the isolation
of Streptococcus pneumoniae and Erysipelothrix rhusiopathiae.