Azide Blood Agar Base, HiVeg with Blood

[所属分类:培养基配方] [发布时间:2019-11-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azide Blood Agar Base, HiVeg with Blood
Composition per liter:
Agar............................................................................................15.0g
Plant special peptone ..................................................................10.0g
NaCl..............................................................................................5.0g
Plant extract..................................................................................3.0g
NaN 3 .............................................................................................0.2g
Sheep blood, defibrinated........................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium without sheep blood is available as a premixed
powder from HiMedia.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the isolation and differentiation of streptococci and staphy-
lococci from specimens containing mixed flora and from nonclinical
specimens such as water and sewage.