Azide Citrate Broth

[所属分类:培养基配方] [发布时间:2019-11-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azide Citrate Broth
Composition per liter:
Pancreatic digest of casein..........................................................20.0g
Sodium citrate.............................................................................10.0g
Yeast extract..................................................................................5.0g
Glucose .........................................................................................5.0g
NaCl..............................................................................................5.0g
K2HPO4 .........................................................................................4.0g
KH2PO4 .........................................................................................1.5g
NaN3 ...........................................................................................0.25g
pH 7.0 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–118°C. Prepare double-strength broth for samples larger
than 1.0mL.
Use: For the detection and enrichment of fecal streptococci in water
and sewage.