Azide Dextrose HiVeg Broth

[所属分类:培养基配方] [发布时间:2019-11-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azide Dextrose HiVeg Broth
Composition per liter:
Plant special peptone..................................................................15.0g
Glucose.........................................................................................7.5g
NaCl..............................................................................................7.5g
Plant extract..................................................................................4.5g
NaN3 .............................................................................................0.2g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 12 psi pressure–118°C.
Use: For the detection and enrichment of fecal streptococci in water
and sewage. Also used in the multiple-tube technique as a presumptive
test for the presence of fecal streptococci in water, sewage, food, and
other materials suspected of sewage contamination.