Azide Medium

[所属分类:培养基配方] [发布时间:2019-11-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azide Medium
Composition per liter:
Peptone .......................................................................................10.0g
K2HPO4 ........................................................................................5.0g
Glucose.........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
KH2PO4 ........................................................................................2.0g
NaN3 ...........................................................................................0.25g
Bromcresol Purple solution .......................................................2.0mL
pH 7.2 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 10.0mL:
Bromcresol Purple......................................................................0.16g
Ethanol.....................................................................................10.0mL
Preparation of Bromcresol Purple Solution: Add Bromcresol
Purple to ethanol and bring volume to 10.0mL. Mix thoroughly.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Streptococcus species and Staphylococcus
species from clinical and nonclinical specimens.