Azoarcus Medium (LMG Medium 202)

[所属分类:培养基配方] [发布时间:2019-11-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azoarcus Medium
(LMG Medium 202)
Composition per liter:
Solution A..............................................................................750.0mL
Phosphate buffer solution ......................................................250.0mL
pH 6.8 ± 0.2 at 25°C
Solution A:
Composition per 750.0mL:
Malic acid .....................................................................................5.0g
KOH..............................................................................................4.5g
MgSO4 ·7H2O................................................................................0.2g
NaCl..............................................................................................0.1g
CaCl2 ........................................................................................20.0mg
MnSO4·H2O.............................................................................10.0mg
Na2MoO4·2H2O .........................................................................2.0mg
Ferric EDTA solution...............................................................10.0mL
Ferric EDTA Solution:
Composition per liter:
Ferric EDTA..............................................................................0.066g
Preparation of Ferric EDTA Solution: Add ferric EDTA to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Solution A: Add 5.0g malic acid to 500.0mL dis-
tilled/deionized water. Adjust pH to 7.0 with KOH (approximate
amount of 4.5g). Add other components. Mix thoroughly. Bring vol-
ume to 750.0mL. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C.
Phosphate Buffer Solution:
Composition per liter:
Na2HPO4 ·2H2O.............................................................................5.8g
KH2PO4 .........................................................................................4.5g
Preparation of Phosphate Buffer Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 25°C.
Preparation of Medium: Aseptically combine 750.0mL sterile so-
lution A with 250.0mL sterile phosphate buffer solution. Aseptically
distribute to sterile tubes or flasks.
Use: For the cultivation of Azoarcus indigens.