Azoarcus VM Medium (LMG Medium 252)

[所属分类:培养基配方] [发布时间:2019-11-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azoarcus VM Medium
(LMG Medium 252)
Composition per liter:
Agar ............................................................................................15.0g
Beef extract...................................................................................3.0g
DL -malic acid ................................................................................2.5g
KOH..............................................................................................2.5g
KH2PO4 .........................................................................................1.5g
NaCl..............................................................................................1.1g
K2HPO4 .........................................................................................1.0g
Yeast extract..................................................................................1.0g
MgSO4 ·7H2O................................................................................0.2g
CaCl2 .............................................................................................0.2g
Fe EDTA..................................................................................66.0mg
MnSO4 ·H2O.............................................................................10.0mg
Na2MoO4 ·2H2O.........................................................................2.0mg
Biotin.........................................................................................0.1mg
NH4Cl ........................................................................................0.5mg
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Azospira oryzae and
Azonexus fungiphilus.