Azospirillum Medium

[所属分类:培养基配方] [发布时间:2019-11-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azospirillum Medium
Composition per liter:
Sodium malate.....................................................................5.0g
Agar..............................................................................1.75g
KH2PO4 ...........................................................................0.4g
MgSO4 ·7H2O.......................................................................0.2g
K2HPO4 ............................................................................0.1g
NaCl................................................................................0.1g
CaCl2 ·2H2O ........................................................................0.02g
FeCl3 ..............................................................................0.01g
Na2MoO4 ·2H2O.....................................................................2.0mg
Bromthymol Blue solution ........................................................5.0mL
pH 6.8 ± 0.2 at 25°C
Bromthymol Blue Solution:
Composition per 10.0mL:
Bromthymol Blue.........................................................................0.5g
Ethanol.....................................................................................10.0mL
Preparation of Bromthymol Blue Solution: Add Bromthymol
Blue to 10.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Azospirillum species isolated from roots.