Azospirillum Medium

[所属分类:培养基配方] [发布时间:2019-11-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azospirillum Medium
Composition per 950.0mL:
MnSO4·H2O....................................................................2.0g
(NH4)2SO4 ......................................................................1.0g
K2HPO4 .........................................................................0.25g
MgSO4·7H2O.....................................................................0.2g
NaCl.............................................................................0.1g
Yeast extract....................................................................0.05g
CaCl2·2H2O ....................................................................0.02g
FeSO4·7H2O......................................................................0.01g
Bromthymol Blue..................................................................25.0mg
Na2MoO4 ·2H2O...................................................................1.0mg
Biotin...........................................................................0.1mg
Glucose solution................................................................25.0mL
Sodium malate solution...........................................................25.0mL
Bromthymol Blue solution ........................................................5.0mL
pH 7.1 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
D- Glucose........................................................................20.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Sodium Malate Solution:
Composition per 100.0mL:
Sodium malate.....................................................................20.0g
Preparation of Sodium Malate Solution : Add sodium malate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Bromthymol Blue Solution:
Composition per 100.0mL:
Bromthymol Blue...................................................................0.5g
Preparation of Bromthymol Blue Solution:  Add Bromthymol
Blue to 100.0mL of 0.2N KOH. Mix thoroughly.
Preparation of Medium: Add components, except glucose solu-
tion and sodium malate solution, to distilled/deionized water and bring
volume to 950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature. Aseptically add 25.0mL of
sterile glucose solution and 25.0mL of sterile sodium malate solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Azospirillum species.