Azospirillum Medium with 0.17% Agar

[所属分类:培养基配方] [发布时间:2019-11-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azospirillum Medium with 0.17% Agar
Composition per liter:
Malic acid .....................................................................5.0g
Agar ...........................................................................1.75
K2HPO4 .........................................................................0.5g
FeSO4 ·7H2O...................................................................0.5g
MgSO4 ·7H2O...................................................................0.2g
NaCl..........................................................................0.1g
CaCl2 ·2H2O..................................................................0.02g
MnSO4 ·H2O...................................................................0.01g
Na2MoO4 ·2H2O ...............................................................2.0mg
Bromthymol Blue ...............................................................2.0mg
Potassium hydroxide solution..................................................50.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Potassium Hydroxide Solution:
Composition per 50.0mL:
KOH............................................................................4.0g
Preparation of Potassium Hydroxide Solution:  Add KOH to
distilled/deionized water and bring volume to 50.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except potassium hy-
droxide solution, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add potassium hydroxide solution,.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the enrichment and cultivation of Azospirillum spp.