Azotobacter Basal Agar

[所属分类:培养基配方] [发布时间:2019-11-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azotobacter Basal Agar
Composition per liter:
Agar ............................................................................................15.0g
K2HPO4 .........................................................................................1.0g
MgSO4 ·7H2O................................................................................0.2g
NaCl..............................................................................................0.2g
FeSO4 ·7H2O...............................................................................5.0mg
Soil extract.............................................................................100.0mL
pH 7.2 ± 0.2 at 25°C
Soil Extract:
Composition per 200.0mL:
African Violet soil.........................................................................0.5g
Na2CO3 .........................................................................................0.5g
Preparation of Soil Extract: Add components to tap water and
bring volume to 200.0mL. Autoclave for 60 min at 15 psi pressure–
121°C. Filter through Whatman filter paper.
Preparation of Medium: Add components, including filtered soil
extract, to tap water and bring volume to 1.0L. Mix thoroughly. Gently
heat and bring to boiling. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave
in tubes.
Use: For the cultivation of a variety of bacteria, including Azomonas
species, Azotobacter species, and others when a carbon source is
added.