Azotobacter Basal Broth

[所属分类:培养基配方] [发布时间:2019-11-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azotobacter Basal Broth
Composition per liter:
K2HPO4 .........................................................................................1.0g
MgSO4 ·7H2O................................................................................0.2g
NaCl..............................................................................................0.2g
FeSO4 ·7H2O...............................................................................5.0mg
Soil extract.............................................................................100.0mL
pH 7.2 ± 0.2 at 25°C
Soil Extract:
Composition per 200.0mL:
African Violet soil.........................................................................0.5g
Na2CO3 .........................................................................................0.5g
Preparation of Soil Extract: Add components to tap water and
bring volume to 200.0mL. Autoclave for 60 min at 15 psi pressure–
121°C. Filter through Whatman filter paper.
Preparation of Medium: Add components, including filtered soil ex-
tract, to tap water and bring volume to 1.0L. Mix thoroughly. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of a variety of bacteria, including Azomonas
species, Azotobacter species, and others when a carbon source is
added.