Azotobacter paspali Medium

[所属分类:培养基配方] [发布时间:2019-11-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azotobacter paspali Medium
Composition per liter:
Agar .............................................................................20.0g
Sucrose...........................................................................20.0g
CaCO3 .............................................................................1.0g
MgSO4 ·7H2O.......................................................................0.2g
KH2PO4 ............................................................................0.15g
K2HPO4 ............................................................................0.05g
CaCl2 .............................................................................0.02g
Na2MoO4 ·2H20.....................................................................2.0mg
Bromthymol Blue solution ......................................................10.0mL
FeCl 3 (10% solution)...........................................................0.1mL
pH 7.0 ± 0.2 at 25°C
Bromthymol Blue Solution:
Composition per 10.0mL:
Bromthymol Blue...............................................................0.5g
Ethanol......................................................................10.0mL
Preparation of Bromthymol Blue Solution: Add Bromthymol
Blue to 10.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Azotobacter paspali.