Azotobacter Supplement (ATCC Medium 11)

[所属分类:培养基配方] [发布时间:2019-11-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azotobacter Supplement
(ATCC Medium 11)
Composition per liter:
Agar..............................................................................15.0g
K2HPO4 ............................................................................1.0g
MgSO4 ·7H2O.......................................................................0.2g
NaCl...............................................................................0.2g
FeSO4 ·7H2O.......................................................................5.0mg
Soil extract.....................................................................100.0mL
Glucose solution...............................................................100.0mL
pH 7.6 ± 0.2 at 25°C
Soil Extract:
Composition per 200.0mL:
African Violet soil..................................................................0.5g
Na 2 CO 3 ...........................................................................0.5g
Preparation of Soil Extract: Add components to tap water and
bring volume to 200.0mL. Autoclave for 60 min at 15 psi pressure–
121°C. Filter through Whatman filter paper.
Glucose Solution:
Composition per 100.0mL:
Glucose...............................................................................20.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except glucose solu-
tion, to tap water and bring volume to 900.0mL. Mix thoroughly. Ad-
just pH to 7.6. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C. Aseptically add 100.0mL of sterile glucose solution. Mix
thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Azomonas agilis and Azotobacter chroo-
coccum.