Azotobacter Supplement (ATCC Medium 12)

[所属分类:培养基配方] [发布时间:2019-11-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azotobacter Supplement
(ATCC Medium 12)
Composition per liter:
Agar..............................................................................15.0g
K2HPO4 ............................................................................1.0g
MgSO4 ·7H2O.......................................................................0.2g
NaCl...............................................................................0.2g
FeSO4 ·7H2O......................................................................5.0mg
Soil extract....................................................................100.0mL
Mannitol solution..............................................................100.0mL
pH 7.6 ± 0.2 at 25°C
Soil Extract:
Composition per 200.0mL:
African Violet soil....................................................................0.5g
Na2CO3 .................................................................................0.5g
Preparation of Soil Extract: Add components to distilled/deion-
ized water and bring volume to 200.0mL. Autoclave for 60 min at 15
psi pressure–121°C. Filter through Whatman filter paper.
Mannitol Solution:
Composition per 100.0mL:
Mannitol.................................................................................20.0g
Preparation of Mannitol Solution:  Add mannitol to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except mannitol solution,
to tap water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to
7.6. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 100.0mL of sterile mannitol solution. Mix thoroughly.
Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Azotobacter species and Azomonas species.