Azotobacter Supplement (ATCC Medium 13)

[所属分类:培养基配方] [发布时间:2019-11-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azotobacter Supplement
(ATCC Medium 13)
Composition per liter:
Agar .............................................................................15.0g
K2HPO4 ............................................................................1.0g
MgSO4 ·7H2O........................................................................0.2g
NaCl................................................................................0.2g
FeSO4 ·7H2O.......................................................................5.0mg
Soil extract......................................................................100.0mL
Glucose solution ................................................................100.0mL
pH 6.0 ± 0.2 at 25°C
Soil Extract:
Composition per 200.0mL:
African Violet soil...................................................................0.5g
Na2CO3 ...............................................................................0.5g
Preparation of Soil Extract: Add components to tap water and
bring volume to 200.0mL. Autoclave for 60 min at 15 psi pressure–
121°C. Filter through Whatman filter paper.
Glucose Solution:
Composition per 100.0mL:
Glucose ..............................................................................20.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except glucose solution,
to tap water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to
6.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add sterile glucose solution. Mix thoroughly. Pour into sterile
Petri dishes or leave in tubes.
Use: For the cultivation of Beijerinckia species.