B Broth (Medium for Ureaplasma)

[所属分类:培养基配方] [发布时间:2019-11-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

B Broth
(Medium for Ureaplasma)
Composition per 100.25mL:
Yeast extract...............................................................0.1g
GHL (Glycyl- L –histidyl- L –lysine)....................................2.0μg
PPLO broth without Crystal Violet.........................................50.0mL
Horse serum, not inactivated .............................................10.0mL
Bromthymol Blue (0.4% solution)............................................1.0mL
Urea solution.............................................................0.25mL
pH 6.0 ± 0.2 at 25°C
PPLO Broth without Crystal Violet:
Composition per 50.0mL:
Beef heart, infusion from....................................................1.62g
Peptone......................................................................0.32g
NaCl.........................................................................0.16g
Source: PPLO broth without Crystal Violet is available as a premixed
powder from BD Diagnostic Systems.
Preparation of PPLO Broth without Crystal Violet: Add
components to distilled/deionized water and bring volume to 50.0mL.
Mix thoroughly.
Urea Solution:
Composition per 10.0mL:
Urea.........................................................................1.0g
Preparation of Urea Solution: Add urea to distilled/deionized wa-
ter and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components—except GHL, urea so-
lution, and horse serum—to double glass-distilled water and bring vol-
ume to 90.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. To
90.0mL of the sterile medium, aseptically add 2.0μg of GHL, 10.0mL
of horse serum, and 0.25mL of sterile urea solution. Mix thoroughly.
Aseptically distribute into tubes or flasks.
Use:  For the cultivation and maintenance of Ureaplasma urealyticum
and other Ureaplasma species.