B/1t 7 A Medium

[所属分类:培养基配方] [发布时间:2019-11-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

B/1t 7 A Medium
Composition per liter:
Agar ............................................................................20.0g
K2HPO4 ...........................................................................7.0g
KH2PO4 ...........................................................................3.0g
Glucose ..........................................................................2.0g
(NH4)2SO4 ........................................................................1.0g
MgSO4·7H2O.......................................................................0.1g
CaCl2 ·2H2O......................................................................0.01g
Indole............................................................................0.01g
FeSO4 ·7H20......................................................................0.5mg
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Escherichia coli and other
bacteria.