B 12 Assay HiVeg Medium (Vitamin B 12 Assay HiVeg Medium)

[所属分类:培养基配方] [发布时间:2019-11-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

B 12 Assay HiVeg Medium
(Vitamin B 12 Assay HiVeg Medium)
Composition per liter:
Glucose........................................................................40.0g
Sodium acetate................................................................20.0g
Plant hydrolysate .............................................................15.0g
Ascorbic acid...................................................................4.0g
Polysorbate 80 .................................................................2.0g
K2HPO4 .........................................................................1.0g
KH2PO4 .........................................................................1.0g
DL- Tryptophan..................................................................0.4g
MgSO4 ·7H2O....................................................................0.4g
L- Cysteine .....................................................................0.4g
Asparagine........................................................................0.2g
Adenine sulfate..................................................................0.02g
FeSO4 ...........................................................................0.02g
Guanine hydrochloride ...........................................................0.02g
MnSO4 ...........................................................................0.02g
NaCl.............................................................................0.02g
Uracil............................................................................0.02g
Xanthine..........................................................................0.02g
Pyridoxal·HCl....................................................................4.0mg
Pyridoxine·HCl...................................................................4.0mg
Niacin............................................................................2.0mg
p-Aminobenzoic acid..............................................................2.0mg
Riboflavin........................................................................1.0mg
Thymine·HCl......................................................................1.0mg
Calcium pantothenate ..............................................................1.0mg
Pyridoxamine·HCl..................................................................0.8mg
Folic acid ........................................................................0.2mg
Biotin.............................................................................0.01mg
pH 6.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the determination of the vitamin B 12 content of pharmaceuti-
cal products and other materials. Lactobacillus leischmanii ATCC
7830 is used as a test organism. A standard curve can be generated by
adding known concentrations of cyanocobalamin and measuring the
growth response turbidimetrically at 530 nm.