B 12 Assay Medium

[所属分类:培养基配方] [发布时间:2019-11-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

B 12 Assay Medium
Composition per liter:
Glucose..........................................................................20.5g
Lactose..........................................................................20.0g
Amino acids, vitamin-free casamino acids.................................15.0g
Sodium acetate...................................................................10.0g
K2HPO4 ...........................................................................2.5g
Polysorbate 80 ...................................................................2.0g
Ascorbic acid.....................................................................1.0g
L- Arginine........................................................................0.5g
L- Histidine......................................................................0.25g
L- Phenylalanine...................................................................0.25g
L- Valine..........................................................................0.25g
L- Asparagine.......................................................................0.2g
MgSO4 ·7H2O........................................................................0.2g
Mercaptoacetic acid................................................................0.13g
Calcium pantothenate ...............................................................0.1g
L- Tryptophan.......................................................................0.1g
MnSO4 .............................................................................0.08g
Adenine............................................................................0.04g
Guanine.............................................................................0.04g
Thymine............................................................................0.04g
Uracil ............................................................................0.04g
(NH4)2SO4 ·FeSO4 ·6H2O...........................................................0.03g
KCN................................................................................5.0mg
Pyridoxal·HCl.....................................................................1.0mg
Niacin.............................................................................1.0mg
Riboflavin ........................................................................1.0mg
Thiamine·HCl .....................................................................0.5mg
p-Aminobenzoic acid................................................................0.5mg
Folic acid........................................................................0.05mg
pH 6.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Cyanide is toxic.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Allow precipitate to settle
out. Distribute supernatant into tubes in 5.0mL volumes. Add standard
solution or test solutions to each tube. Adjust the volume of each tube
to 10.0mL with distilled/deionized water. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the determination of the vitamin B 12 content of pharmaceuti-
cal products and other materials. Lactobacillus leischmanii ATCC
7830 is used as a test organism. A standard curve can be generated by
adding known concentrations of cyanocobalamin and measuring the
growth response turbidimetrically at 530 nm.