B 12 Culture Agar, USP

[所属分类:培养基配方] [发布时间:2019-11-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

B 12 Culture Agar, USP
Composition per liter:
Agar ..........................................................................15.0g
Glucose .......................................................................10.0g
Proteose peptone No. 3..........................................................7.5g
Yeast extract...................................................................7.5g
KH2PO4 .........................................................................2.0g
Polysorbate 80..................................................................0.1g
Tomato juice.................................................................100.0mL
pH 6.8 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C. Cool tubes in an upright position.
Use: For the cultivation and maintenance of Lactobacillus leischmannii
ATCC 7830 to be used as the test organism in the Vitamin B 12 assay
according to the USP.