B 12 Inoculum Broth, USP

[所属分类:培养基配方] [发布时间:2019-11-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

B 12  Inoculum Broth, USP
Composition per liter:
Glucose .................................................................10.0g
Proteose peptone No. 3....................................................7.5g
Yeast extract.............................................................7.5g
K2HPO4 ...................................................................2.0g
Polysorbate 80 ...........................................................0.1g
Tomato juice ..........................................................100.0mL
pH 6.8 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the preparation of inoculum cultures of Lactobacillus leis-
chmanii ATCC 7830, which is used as the test organism in the vitamin
B 12 assay according to the USP.