Baar’s Medium for Sulfate Reducers

[所属分类:培养基配方] [发布时间:2019-11-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Baar’s Medium for Sulfate Reducers
Composition per liter:
Sodium lactate..................................................................3.5g
MgSO4 ·7H2O....................................................................2.0g
K2HPO4 .........................................................................1.0g
CaSO4 ..........................................................................1.0g
NH4Cl ..........................................................................0.5g
Ferrous ammonium sulfate solution ........................................10.0mL
Yeast extract solution........................................................10.0mL
pH 7.5 ± 0.2 at 25°C
Ferrous Ammonium Sulfate Solution:
Composition per 10.0mL:
Fe(NH4)2(SO4)2 ...............................................................0.5g
Preparation of Ferrous Ammonium Sulfate Solution: Add
Fe(NH4)2(SO4 ) 2 to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract..................................................................1.0g
Preparation of Yeast Extract Solution: Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except ferrous ammoni-
um sulfate solution and yeast extract solution, to tap water and bring vol-
ume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asep-
tically add 10.0mL of sterile ferrous ammonium sulfate solution and sterile
yeast extract solution. Aseptically distribute into tubes or flasks.
Use:  For the cultivation and maintenance of Desulfotomaculum nigrifi-
cans.