Baar’s Medium for Sulfate Reducers, Modified

[所属分类:培养基配方] [发布时间:2019-11-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Baar’s Medium for Sulfate Reducers, Modified
Composition per 1020.0mL:
Component I ................................................................400.0mL
Component III...............................................................400.0mL
Component II................................................................200.0mL
Ferrous ammonium sulfate solution ........................................20.0mL
pH 7.5 ± 0.2 at 25°C
Component I:
Composition per 400.0mL:
Sodium citrate..................................................................5.0g
MgSO4 ..........................................................................2.0g
CaSO4 ..........................................................................1.0g
NH4Cl ..........................................................................1.0g
Preparation of Component I: Add components to distilled/deion-
ized water and bring volume to 400.0mL. Mix thoroughly. Adjust pH
to 7.5. Autoclave for 15 min at 15 psi pressure–121°C.
Component II:
Composition per 200.0mL:
K2HPO4 ............................................................................0.5g
Preparation of Component II: Add K 2 HPO 4 to distilled/deion-
ized water and bring volume to 200.0mL. Mix thoroughly. Adjust pH
to 7.5. Autoclave for 15 min at 15 psi pressure–121°C.
Component III:
Composition per 400.0mL:
Sodium lactate ....................................................................3.5g
Yeast extract......................................................................1.0g
Preparation of Component III: Add components to distilled/de-
ionized water and bring volume to 400.0mL. Mix thoroughly. Adjust
pH to 7.5. Autoclave for 15 min at 15 psi pressure–121°C.
Ferrous Ammonium Sulfate Solution:
Composition per 20.0mL:
Fe(NH4)2(SO4)2 ......................................................................1.0g
Preparation of Ferrous Ammonium Sulfate Solution: Add
Fe(NH4)2(SO4)2 to distilled/deionized water and bring volume to
20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Aseptically combine component I, com-
ponent II, and component III. Mix thoroughly. Distribute 5.0mL vol-
umes into tubes under 97% N 2 + 3% H 2 . Add medium to tubes while
still warm to exclude as much O 2 as possible. Aseptically add 0.1mL
of sterile ferrous ammonium sulfate solution to 5.0mL of medium im-
mediately prior to inoculation.
Use:  For the cultivation and maintenance of Desulfovibrio, Desulfob-
ulbus, Desulfotomaculum, and Thermodesulfobacterium species.