Baar’s Medium for Sulfate Reducers, Modified with 2.5% Sodium Chloride

[所属分类:培养基配方] [发布时间:2019-11-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Baar’s Medium for Sulfate Reducers,
Modified with 2.5% Sodium Chloride
Composition per 1020.0mL:
Component I ..........................................................................400.0mL
Component III........................................................................400.0mL
Component II.........................................................................200.0mL
Ferrous ammonium sulfate solution ........................................20.0mL
pH 7.5 ± 0.2 at 25°C
Component I:
Composition per 400.0mL:
NaCl............................................................................................25.0g
Sodium citrate...............................................................................5.0g
MgSO4 ..........................................................................................2.0g
CaSO4 ...........................................................................................1.0g
NH4Cl ...........................................................................................1.0g
Preparation of Component I: Add components to distilled/deion-
ized water and bring volume to 400.0mL. Mix thoroughly. Adjust pH
to 7.5. Autoclave for 15 min at 15 psi pressure–121°C.
Component II:
Composition per 200.0mL:
K2HPO4 .........................................................................................0.5g
Preparation of Component II: Add K 2 HPO 4 to distilled/deion-
ized water and bring volume to 200.0mL. Mix thoroughly. Adjust pH
to 7.5. Autoclave for 15 min at 15 psi pressure–121°C.
Component III:
Composition per 400.0mL:
Sodium lactate...............................................................................3.5g
Yeast extract..................................................................................1.0g
Preparation of Component III: Add components to distilled/de-
ionized water and bring volume to 400.0mL. Mix thoroughly. Adjust
pH to 7.5. Autoclave for 15 min at 15 psi pressure–121°C.
Ferrous Ammonium Sulfate Solution:
Composition per 20.0mL:
Fe(NH4)2(SO4)2 .............................................................................1.0g
Preparation of Ferrous Ammonium Sulfate Solution: Add
Fe(NH4)2(SO4) 2 to distilled/deionized water and bring volume to
20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Aseptically combine component I, compo-
nent II, and component III. Mix thoroughly. Distribute 5.0mL volumes
into tubes under 97% N 2 + 3% H 2 . Add medium to tubes while still warm
to exclude as much O 2 as possible. Aseptically add 0.1mL of sterile ferrous
ammonium sulfate solution to 5.0mL of medium immediately prior to in-
oculation.
Use:  For the cultivation of Desulfovibrio africanus and other Desulfovi-
brio species that prefer 2.5% NaCl.