Bacillus acidocaldarius Agar

[所属分类:培养基配方] [发布时间:2019-11-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus acidocaldarius Agar
Composition per liter:
Solution A....................................................................500.0mL
Solution B....................................................................500.0mL
pH 3.0–4.0 at 25°C
Solution A:
Composition per 500.0mL:
KH2PO4 ..........................................................................3.0g
Yeast extract....................................................................1.0g
Glucose ..........................................................................1.0g
MgSO4 ·7H2O......................................................................0.5g
CaCl2 ·2H2O.....................................................................0.25g
(NH4)2SO4 ........................................................................0.2g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 500.0mL. Adjust pH to 3.0–4.0. Mix thor-
oughly. Autoclave for 10 min at 15 psi pressure–121°C. Cool to 50°–
55°C.
Solution B:
Composition per 500.0mL:
Agar ...............................................................................30.0g
Preparation of Solution B:  Add agar to distilled/deionized water
and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C.
Preparation of Medium: Aseptically mix 500.0mL of solution A
and 500.0mL of solution B. Mix thoroughly. Aseptically adjust pH to
3.0–4.0. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Bacillus acidocaldarius.