Bacillus acidocaldarius Agar

[所属分类:培养基配方] [发布时间:2019-11-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus acidocaldarius Agar
Composition per liter:
Solution A......................................................................500.0mL
Solution B......................................................................500.0mL
pH 3.5 ± 0.5 at 25°C
Solution A:
Composition per 500.0mL:
Yeast extract.....................................................................1.0g
KH2PO4 ............................................................................0.6g
MgSO4 ·7H2O.......................................................................0.5g
CaCl2 ·2H2O ......................................................................0.25g

(NH4)2 ·SO4 .......................................................................0.2g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Bring pH to 3.5.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Solution B:
Composition per 500.0mL:
Agar.................................................................................20.0g
Glucose..............................................................................1.0g
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C.
Preparation of Medium: Aseptically combine 500.0mL of solu-
tion A with 500.0mL of solution B. Mix thoroughly. Pour into sterile
Petri dishes or aseptically distribute into sterile tubes.
Use: For the cultivation and maintenance of Alicyclobacillus acido-
caldarius.