Bacillus acidoterrestris Agar

[所属分类:培养基配方] [发布时间:2019-11-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus acidoterrestris Agar
Composition per 1001.0mL:
Solution A..................................................................500.0mL
Solution C..................................................................500.0mL
Solution B (Trace elements solution SL-6) ...............................1.0mL
pH 4.0 ± 0.2 at 25°C
Solution A:
Composition per 500.0mL:
Glucose.........................................................................5.0g
KH2PO4 .........................................................................3.0g
Yeast extract...................................................................2.0g
MgSO4 ·7H2O....................................................................0.5g
CaCl2 ·2H2O ..................................................................0.25g
(NH4)2SO4 ......................................................................0.2g
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 4.0.
Autoclave for 15 min at 15 psi pressure–121°C.
Solution C:
Composition per 500.0mL:
Agar............................................................................15.0g
Preparation of Solution C: Add agar to distilled/deionized water
and bring volume to 500.0mL. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Solution B (Trace Elements Solution SL-6):
Composition per liter:
MnCl2 ·4H2O ....................................................................0.5g
H3BO3 ...........................................................................0.3g
CoCl2 ·6H2O.....................................................................0.2g
ZnSO4 ·7H2O.....................................................................0.1g
Na2MoO 4 ·2H2O.................................................................0.03g
NiCl2 ·6H2O....................................................................0.02g
CuCl2 ·2H2O....................................................................0.01g
Preparation of Solution B (Trace Elements Solution SL-6):
Add components to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Aseptically combine 500.0mL of sterile
solution A, 500.0mL of sterile solution C, and 1.0mL of sterile solution
B. Mix thoroughly. Pour into sterile Petri dishes or distribute into ster-
ile tubes.
Use:  For the cultivation and maintenance of Bacillus acidoterrestris,
Alicyclobacillus acidoterrestris, and Alicyclobacillus cycloheptanicus.