Bacillus Agar

[所属分类:培养基配方] [发布时间:2019-11-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus Agar
Composition per liter:
Agar ..............................................................................20.0g
(NH4)2SO4 ..........................................................................1.3g
Glucose ............................................................................1.0g
Yeast extract.......................................................................1.0g
KH2PO4 ............................................................................0.37g
MgSO4·7H2O........................................................................0.25g
CaCl2·2H2O........................................................................0.07g
FeCl3 .............................................................................0.02g
pH 4.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Adjust pH to 3.5. Prepare a separate agar solution by
adding 20.0g/500.0mL of distilled/deionized water. Autoclave solu-
tions separately for 15 min at 15 psi pressure–121°C. Cool to 50°–
55°C. Aseptically combine both solutions. This procedure avoids acid
hydrolysis of the agar. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of acidophilic Bacillus species such as Bacil-
lus acidocaldarius.