Bacillus Agar, Modified

[所属分类:培养基配方] [发布时间:2019-11-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus Agar, Modified
Composition per liter:
Agar.................................................................................20.0g
Glucose...............................................................................1.0g
Yeast extract.........................................................................1.0g
KH2PO4 ...............................................................................0.6g
MgSO4 ·7H2O..........................................................................0.5g
CaCl2 ·2H2O ........................................................................0.25g
(NH4) 2SO4 ...........................................................................0.2g
pH 3.0–4.0 at 25°C
Preparation of Medium: Add components, except agar and glu-
cose, to distilled/deionized water and bring volume to 500.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 3.5. Prepare
a separate agar and glucose solution by adding 20.0g of agar and 1.0g
of glucose to 500.0mL of distilled/deionized water. Autoclave solu-
tions separately for 15 min at 15 psi pressure–121°C. Cool to 50°–
55°C. Aseptically combine both solutions. This procedure avoids acid
hydrolysis of the agar. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of acidophilic Bacillus species such as Bacil-
lus acidocaldarius.