Bacillus benzoevorans Agar

[所属分类:培养基配方] [发布时间:2019-11-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus benzoevorans Agar
Composition per liter:
Modified Palleroni and Doudoroff mineral base medium.....450.0mL
Enriched Cytophaga agar.........................................450.0mL
Sodium benzoate solution........................................100.0mL
pH 7.0 ± 0.2 at 25°C
Modified Palleroni and Doudoroff Mineral Base Medium:
Composition per 500.0mL:
Agar ....................................................................15.0g
Na2HPO4 ·12H2O...........................................................6.0g
KH2PO4 ...................................................................2.4g
NH4·Cl ..................................................................1.0g
MgSO4 ·7H2O...............................................................0.5g
CaCl2 ·6H2O..............................................................0.01g
FeCl3 ·6H2O..............................................................0.01g
Preparation of Modified Palleroni and Doudoroff Mineral
Base Medium: Add components to distilled/deionized water and
bring volume to 450.0mL. Mix thoroughly. Adjust pH to 7.2. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C.
Enriched Cytophaga Agar:
Composition per 500.0mL:
Agar ........................................................................15.0g
Pancreatic digest of casein...................................................0.5g
Beef extract..................................................................0.5g
Yeast extract.................................................................0.5g
Sodium acetate................................................................0.2g
Preparation of Enriched Cytophaga Agar:  Add components to
distilled/deionized water and bring volume to 450.0mL. Mix thorough-
ly. Adjust pH to 6.8. Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C.
Sodium Benzoate Solution:
Composition per 100.0mL:
Sodium benzoate...............................................................5.0g
Preparation of Sodium Benzoate Solution:  Add sodium benzo-
ate to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Aseptically combine 450.0mL of modi-
fied Palleroni and Doudoroff mineral base medium, 450.0mL of en-
riched Cytophaga agar, and 100.0mL sodium benzoate solution. Mix
thoroughly. Pour into sterile Petri dishes or aseptically distribute into
sterile tubes.
Use:  For the cultivation of Bacillus benzoevorans.