Bacillus Broth

[所属分类:培养基配方] [发布时间:2019-11-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus Broth
Composition per liter:
(NH4)2SO4 .......................................................................1.3g
Glucose .........................................................................1.0g
Yeast extract....................................................................1.0g
KH2PO4 .........................................................................0.37g
MgSO4 ·7H2O....................................................................0.25g
CaCl2 ·2H2O ...................................................................0.07g
FeCl3 ..........................................................................0.02g
pH 4.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Adjust pH to 4.0 with 10N H 2 SO 4 . Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of acidophilic Bacillus species such as Bacil-
lus acidocaldarius.